Part:BBa_K1641042

Fusion protein of Cre-EGFP, with RBS at the beginning

This is a sequence of RBS (BBa_B0034) followed by a fusion protein of Cre-EGFP. The part is for expression of Cre-EGFP, which aims to overturn our invertase modules in our Micro-timer.

Cre (BBa_K1179058) is a most commonly used recombinase with invertase activity that turns the sequence between two anti-parallel LoxP sites up-side-down. An EGFP fusion at the C-term of Vcre will not significantly interfere the enzymatic activity, and a flexible chain of 8 AA is added between the two parts to further avoid side-effect like misfolding. Unlike other invertase module in Micro-timer of SYSU-CHINA in 2015, this part does not have ssra-tag, and thus has better expression efficiency and enzymatic activity.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 287
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]